The Polymerase Chain Reaction is a powerful tool in molecular biology that allows researchers to amplify specific segments of DNA. The basic Polymerase Chain Reaction process involves denaturation, annealing, and extension, and can be used in a wide range of applications, from medical research to forensic science. Variations of PCR, such as RT-PCR and real-time PCR, allow researchers to amplify RNA sequences and monitor the progress of the reaction in real-time.
A laboratory procedure called Polymerase Chain Reaction, or polymerase chain reaction, is used to replicate a DNA fragment several times. A specific DNA target may be amplified, or copied, using the highly accurate PCR method from a mixture of DNA molecules. Primers are two quick DNA sequences that are created to attach to the beginning and end of the DNA target. The target DNA template is then put to a tube containing primers, free nucleotides, and an enzyme called DNA polymerase. The combination is then placed in a PCR machine to execute PCR. The sample's temperature is raised and lowered by the PCR equipment in automated, preprogrammed stages.
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